Microbial Dehalorespiration with 1,1,1-Trichloroethane

Author:

Sun Baolin1,Griffin Benjamin M.12,Ayala-del-Rı́o Héctor L.12,Hashsham Syed A.13,Tiedje James M.12

Affiliation:

1. Center for Microbial Ecology,

2. Department of Microbiology and Molecular Genetics,

3. Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824–1325, USA.

Abstract

1,1,1-Trichloroethane (TCA) is a ubiquitous environmental pollutant because of its widespread use as an industrial solvent, its improper disposal, and its substantial emission to the atmosphere. We report the isolation of an anaerobic bacterium, strain TCA1, that reductively dechlorinates TCA to 1,1-dichloroethane and chloroethane. Strain TCA1 required H 2 as an electron donor and TCA as an electron acceptor for growth, indicating that dechlorination is a respiratory process. Phylogenetic analysis indicated that strain TCA1 is related to gram-positive bacteria with low DNA G+C content and that its closest relative is Dehalobacter restrictus , an obligate H 2 -oxidizing, chloroethene-respiring bacterium.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference28 articles.

1. Agency for Toxic Substances and Disease Registry ToxFAQs for 1 1 1-Trichloroethane (September 1996; www.atsdr.cdc.gov/tfacts70.html).

2. The Groundwater Supply Survey

3. New Observational Constraints for Atmospheric Hydroxyl on Global and Hemispheric Scales

4. Federal Register Fed. Regist. 54 22062 (1989).

5. United Nations Environment Programme Production and Consumption of Ozone Depleting Substances 1986–1998 (October 1999; www.unep.org/ozone/datareport99.shtml).

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