Evidence that the Fosfomycin-Producing Epoxidase, HppE, Is a Non–Heme-Iron Peroxidase-Reference-Cited by-同舟云学术

Evidence that the Fosfomycin-Producing Epoxidase, HppE, Is a Non–Heme-Iron Peroxidase

Published:2013-11-22 Issue:6161 Volume:342 Page:991-995
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Wang Chen¹,Chang Wei-chen²,Guo Yisong²,Huang Hui³,Peck Spencer C.⁴,Pandelia Maria E.²,Lin Geng-min³,Liu Hung-wen³,Krebs Carsten¹²,Bollinger J. Martin¹²

Affiliation:

1. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA.

2. Department of Chemistry, The Pennsylvania State University, University Park, PA, USA.

3. Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX, USA.

4. Department of Chemistry and the Howard Hughes Medical Institute, and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Abstract

Just Add Peroxide The HppE enzyme uses iron to catalyze oxidation of an alcohol to an epoxide ring in the biosynthesis of the antibiotic fosfomycin. Because this process is a two-electron oxidation, it has been unclear how the enzyme reduces its presumed oxidative partner O 2 all the way to water. Where do the two extra electrons come from? Wang et al. (p. 991 , published 10 October; see the Perspective by

Raushel

) now show that HppE is actually a peroxidase, and thus reduces H 2 O 2 , for which just two electrons are sufficient. The result expands the structural scope of iron-bearing peroxidase enzymes beyond heme motifs.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference67 articles.

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2. Characterization of a Cys115 to Asp Substitution in the Escherichia coli Cell Wall Biosynthetic Enzyme UDP-GlcNAc Enolpyruvyl Transferase (MurA) That Confers Resistance to Inactivation by the Antibiotic Fosfomycin

3. Protein Purification and Function Assignment of the Epoxidase Catalyzing the Formation of Fosfomycin

4. Purification and Characterization of the Epoxidase Catalyzing the Formation of Fosfomycin from Pseudomonas syringae

5. Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme

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