Capture of a Single Molecule in a Nanocavity-Reference-Cited by-同舟云学术

Capture of a Single Molecule in a Nanocavity

Published:2001-01-26 Issue:5504 Volume:291 Page:636-640
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Gu Li-Qun¹,Cheley Stephen¹,Bayley Hagan¹²

Affiliation:

1. Department of Medical Biochemistry and Genetics, Texas A&M University System Health Science Center, College Station, TX 77843, USA.

2. Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.

Abstract

We describe a heptameric protein pore that has been engineered to accommodate two different cyclodextrin adapters simultaneously within the lumen of a transmembrane β barrel. The volume between the adapters is a cavity of ∼4400 cubic angstroms. Analysis of single-channel recordings reveals that individual charged organic molecules can be pulled into the cavity by an electrical potential. Once trapped, an organic molecule shuttles back and forth between the adapters for hundreds of milliseconds. Such self-assembling nanostructures are of interest for the fabrication of multianalyte sensors and could provide a means to control chemical reactions.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference21 articles.

1. Direct observation of the rotation of F1-ATPase

2. Powering an Inorganic Nanodevice with a Biomolecular Motor

3. Gu L.-Q., Braha O., Conlan S., Cheley S., Bayley H., Nature 398, 686 (1999).

4. Gu L.-Q., et al., Proc. Natl. Acad. Sci. U.S.A. 97, 3959 (2000).

5. Bayley H., Braha O., Gu L.-Q., Adv. Mater. 12, 139 (2000).

Cited by 144 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Nanopore sensing and beyond: Electrochemical systems for optically-coupled single-entity studies, stimulus-responsive gating applications, and point-of-care sensors;Sensors and Actuators Reports;2024-12

2. Stochastic Sensing of Chloride Anions Using an α‐Hemolysin Pore with a semiaza‐Bambusuril Adapter;Angewandte Chemie International Edition;2024-08-29

3. Stochastic Sensing of Chloride Anions Using an α‐Hemolysin Pore with a semiaza‐Bambusuril Adapter;Angewandte Chemie;2024-07-23

4. Switchable Separation Strategy via Host–Guest Locks;Langmuir;2023-02-09

5. Third‐Generation Sequencing of Epigenetic DNA;Angewandte Chemie International Edition;2023-01-25