PiggyBac Transposon Mutagenesis: A Tool for Cancer Gene Discovery in Mice-Reference-Cited by-同舟云学术

PiggyBac Transposon Mutagenesis: A Tool for Cancer Gene Discovery in Mice

Published:2010-11-19 Issue:6007 Volume:330 Page:1104-1107
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Rad Roland¹,Rad Lena¹,Wang Wei¹,Cadinanos Juan¹²,Vassiliou George¹,Rice Stephen¹,Campos Lia S.¹,Yusa Kosuke¹,Banerjee Ruby¹,Li Meng Amy¹,de la Rosa Jorge²,Strong Alexander¹,Lu Dong¹,Ellis Peter¹,Conte Nathalie¹,Yang Fang Tang¹,Liu Pentao¹,Bradley Allan¹

Affiliation:

1. Wellcome Trust Sanger Institute, Genome Campus, Hinxton-Cambridge CB10 1SA, UK.

2. Instituto de Medicina Oncológica y Molecular de Asturias (IMOMA), Avenida Richard Grandío s/n, Oviedo, 33193, Spain.

Abstract

Piggybacking on Cancer Genes Transposons are mobile segments of DNA that can insert in or near important genes to cause mutations that disrupt gene function. Rad et al. (p. 1104 , published online 14 October) adapted a mutagenic transposon called Piggybac, originally derived from a moth, into a tool for discovery of cancer-causing genes in mice. Mobilization of Piggybac in mice was associated with the development of leukemias and solid tumors. In many instances the causative mutations, which were identified by mapping the Piggybac integration sites, were within genes not previously implicated in cancer.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference23 articles.

1. High-throughput insertional mutagenesis screens in mice to identify oncogenic networks

2. Transposon-mediated genome manipulation in vertebrates

3. Molecular Reconstruction of Sleeping Beauty, a Tc1-like Transposon from Fish, and Its Transposition in Human Cells

4. Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells

5. Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system

Cited by 203 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Germ line variant GFI1-36N affects DNA repair and sensitizes AML cells to DNA damage and repair therapy;Blood;2023-12-21

2. Advances in transposable elements: from mechanisms to applications in mammalian genomics;Frontiers in Genetics;2023-11-30

3. Successes and challenges in modeling heterogeneous BRAFV600E mutated central nervous system neoplasms;Frontiers in Oncology;2023-10-18

4. Actionable loss of SLF2 drives B‐cell lymphomagenesis and impairs the DNA damage response;EMBO Molecular Medicine;2023-07-24

5. High-throughput functional screen identifies YWHAZ as a key regulator of pancreatic cancer metastasis;Cell Death & Disease;2023-07-14