Mus81 and converging forks limit the mutagenicity of replication fork breakage-Reference-Cited by-同舟云学术

Mus81 and converging forks limit the mutagenicity of replication fork breakage

Published:2015-08-14 Issue:6249 Volume:349 Page:742-747
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Mayle Ryan¹,Campbell Ian M.¹,Beck Christine R.¹,Yu Yang¹,Wilson Marenda¹,Shaw Chad A.¹,Bjergbaek Lotte²,Lupski James R.¹³⁴,Ira Grzegorz¹

Affiliation:

1. Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.

2. Department of Molecular Biology and Genetics, University of Aarhus, Aarhus 8000, Denmark.

3. Department of Pediatrics, and Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.

4. Texas Children’s Hospital, Houston, TX 77030, USA.

Abstract

How to repair broken replication forks Double-strand breaks in DNA are extremely dangerous to the integrity of our genomes. Most arise from problems encountered by replication forks during duplication of genomic DNA. Break-induced replication is known to use an error-prone DNA polymerase to repair such damage. Mayle et al. show that cells limit error-prone DNA synthesis by preventing the DNA polymerase from inadvertently switching to a related sequence with an incorrect template. The repair of the break is achieved by using a structure-specific nuclease to prevent formation of a long single-stranded region. Science , this issue p. 742

Funder

National Institutes of Health (NIH)

Howard Hughes Medical Institute

Damon Runyon Cancer Research Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference31 articles.

1. Two Mechanisms Produce Mutation Hotspots at DNA Breaks in Escherichia coli

2. Break-Induced Replication Is Highly Inaccurate

3. Template switching during break-induced replication