Rab1 Recruitment of p115 into a cis-SNARE Complex: Programming Budding COPII Vesicles for Fusion-Reference-Cited by-同舟云学术

Rab1 Recruitment of p115 into a cis-SNARE Complex: Programming Budding COPII Vesicles for Fusion

Published:2000-07-21 Issue:5478 Volume:289 Page:444-448
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Allan Bernard B.¹,Moyer Bryan D.¹,Balch William E.¹

Affiliation:

1. Departments of Cell and Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Abstract

The guanosine triphosphatase Rab1 regulates the transport of newly synthesized proteins from the endoplasmic reticulum to the Golgi apparatus through interaction with effector molecules, but the molecular mechanisms by which this occurs are unknown. Here, the tethering factor p115 was shown to be a Rab1 effector that binds directly to activated Rab1. Rab1 recruited p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacted with a select set of COPII vesicle–associated SNAREs (soluble N -ethylmaleimide–sensitive factor attachment protein receptors) to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. We propose that Rab1-regulated assembly of functional effector-SNARE complexes defines a conserved molecular mechanism to coordinate recognition between subcellular compartments.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference69 articles.

1. Tisdale E. J., Bourne J. R., Khosravi-Far R., Der C. J., Balch W. E., J. Cell Biol. 119, 749 (1992).

2. H. Plutner et al. J. Cell Biol. 115 31 (1991).

3. Martinez O., Goud B., Biochim. Biophys. Acta 1404, 101 (1998).

4. Schimmoller F., Simon I., Pfeffer S. R., J. Biol. Chem. 273, 22161 (1998).

5. Bacterially expressed GST-Rab proteins (0.5 mg) were purified on glutathione-Sepharose beads (Pharmacia) loaded with GTPγS or GDP incubated with gel-filtered rat liver cytosol (20 mg) and bound proteins eluted with EDTA as described (11) except that cells were lysed and washed in buffer containing 100 μM GTPγS instead of GDP during preparation of GST-Rab-GTP beads.

Cited by 416 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. β-N-methylamino-L-alanine production, photosynthesis and transcriptional expression in a possible mutation strain and a wild strain of Thalassiosira minima;Journal of Hazardous Materials;2024-09

2. A Humanized Yeast Model for Studying TRAPP Complex Mutations; Proof-of-Concept Using Variants from an Individual with a TRAPPC1-Associated Neurodevelopmental Syndrome;Cells;2024-08-30

3. Insect-transmitted plant virus balances its vertical transmission through regulating Rab1-mediated receptor localization;Cell Reports;2024-08

4. Rab1b facilitates lipid droplet growth by ER–to–lipid droplet targeting of DGAT2;Science Advances;2024-05-31

5. Protein networking: nicotinic acetylcholine receptors and their protein–protein-associations;Molecular and Cellular Biochemistry;2024-05-21