Metagenomic sequencing at the epicenter of the Nigeria 2018 Lassa fever outbreak-Reference-Cited by-同舟云学术

Metagenomic sequencing at the epicenter of the Nigeria 2018 Lassa fever outbreak

Published:2019-01-04 Issue:6422 Volume:363 Page:74-77
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Kafetzopoulou L. E.¹²³^ORCID,Pullan S. T.¹²^ORCID,Lemey P.⁴^ORCID,Suchard M. A.⁵^ORCID,Ehichioya D. U.³⁶^ORCID,Pahlmann M.³⁶,Thielebein A.³⁶,Hinzmann J.³⁶,Oestereich L.³⁶,Wozniak D. M.³⁶^ORCID,Efthymiadis K.⁷,Schachten D.³,Koenig F.³,Matjeschk J.³^ORCID,Lorenzen S.³^ORCID,Lumley S.¹,Ighodalo Y.⁸,Adomeh D. I.⁸,Olokor T.⁸,Omomoh E.⁸,Omiunu R.⁸,Agbukor J.⁸,Ebo B.⁸,Aiyepada J.⁸,Ebhodaghe P.⁸,Osiemi B.⁸,Ehikhametalor S.⁸,Akhilomen P.⁸,Airende M.⁸,Esumeh R.⁸,Muoebonam E.⁸,Giwa R.⁸,Ekanem A.⁸,Igenegbale G.⁸,Odigie G.⁸,Okonofua G.⁸,Enigbe R.⁸,Oyakhilome J.⁸,Yerumoh E. O.⁸,Odia I.⁸,Aire C.⁸,Okonofua M.⁸,Atafo R.⁸,Tobin E.⁸,Asogun D.⁸⁹,Akpede N.⁸,Okokhere P. O.⁸⁹,Rafiu M. O.⁸^ORCID,Iraoyah K. O.⁸,Iruolagbe C. O.⁸,Akhideno P.⁸^ORCID,Erameh C.⁸^ORCID,Akpede G.⁸⁹,Isibor E.⁸,Naidoo D.¹⁰,Hewson R.¹²¹¹¹²^ORCID,Hiscox J. A.²¹³¹⁴,Vipond R.¹²,Carroll M. W.¹²,Ihekweazu C.¹⁵,Formenty P.¹⁰,Okogbenin S.⁸⁹,Ogbaini-Emovon E.⁸,Günther S.³⁶^ORCID,Duraffour S.³⁶^ORCID

Affiliation:

1. Public Health England, National Infection Service, Porton Down, UK.

2. National Institute of Health Research (NIHR), Health Protection Research Unit in Emerging and Zoonotic Infections, University of Liverpool, Liverpool, UK.

3. Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

4. Department of Microbiology and Immunology, Rega Institute, KU Leuven – University of Leuven, Leuven, Belgium.

5. Departments of Biomathematics, Biostatistics, and Human Genetics, University of California, Los Angeles, CA, USA.

6. German Center for Infection Research (DZIF), partner site Hamburg, Germany.

7. Artificial Intelligence Laboratory, Vrije Universiteit Brussel, Brussels, Belgium.

8. Irrua Specialist Teaching Hospital, Irrua, Nigeria.

9. Faculty of Clinical Sciences, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.

10. World Health Organization, Geneva, Switzerland.

11. Faculty of Infectious and Tropical Diseases, Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, UK.

12. Faculty of Clinical Sciences and International Public Health, Liverpool School of Tropical Medicine, Liverpool, UK.

13. Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR), Singapore.

14. Institute of Infection and Global Health, University of Liverpool, Liverpool, UK.

15. Nigeria Centre for Disease Control, Abuja, Nigeria.

Abstract

Mobile detection of Lassa virus Lassa fever is a hemorrhagic viral disease endemic to West Africa. Usually, each year sees only a smattering of cases reported, but hospitalized patients risk a 15% chance of death. Responding to fears that a 10-fold surge in cases in Nigeria in 2018 signaled an incipient outbreak, Kafetzopoulou et al. performed metagenomic nanopore sequencing directly from samples from 120 patients (see the Perspective by Bhadelia). Results showed no strong evidence of a new strain emerging nor of person-to-person transmission; rather, rodent contamination was the main source. To prevent future escalation of this disease, we need to understand what triggers the irruption of rodents into human dwellings. Science , this issue p. 74 ; see also p. 30

Funder

National Science Foundation

European Commission Directorate-General for Research and Innovation

Wellcome Trust

Alexander von Humboldt-Stiftung

Horizon 2020 Framework Programme

Fonds Wetenschappelijk Onderzoek

Onderzoeksraad, KU Leuven

Deutsche Forschungsgemeinschaft

Bundesministeriums für Gesundheit

Bundesministerium für Wirtschaftliche Zusammenarbeit und Entwicklung

National Institute for Health Research