SLAM-seq defines direct gene-regulatory functions of the BRD4-MYC axis-Reference-Cited by-同舟云学术

SLAM-seq defines direct gene-regulatory functions of the BRD4-MYC axis

Published:2018-05-18 Issue:6390 Volume:360 Page:800-805
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Muhar Matthias¹^ORCID,Ebert Anja¹^ORCID,Neumann Tobias¹^ORCID,Umkehrer Christian¹^ORCID,Jude Julian¹^ORCID,Wieshofer Corinna²,Rescheneder Philipp³,Lipp Jesse J.¹,Herzog Veronika A.⁴,Reichholf Brian⁴,Cisneros David A.¹^ORCID,Hoffmann Thomas¹^ORCID,Schlapansky Moritz F.¹^ORCID,Bhat Pooja⁴,von Haeseler Arndt³^ORCID,Köcher Thomas⁵^ORCID,Obenauf Anna C.¹^ORCID,Popow Johannes²,Ameres Stefan L.⁴^ORCID,Zuber Johannes¹⁶^ORCID

Affiliation:

1. Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), 1030 Vienna, Austria.

2. Boehringer Ingelheim–Regional Center Vienna GmbH and Company KG, 1121 Vienna, Austria.

3. Center for Integrative Bioinformatics Vienna, Max F. Perutz Laboratories, University of Vienna and Medical University of Vienna, 1030 Vienna, Austria.

4. Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), VBC, 1030 Vienna, Austria.

5. Vienna Biocenter Core Facilities (VBCF), 1030 Vienna, Austria.

6. Medical University of Vienna, VBC, 1030 Vienna, Austria.

Abstract

Profiling transcription—a SLAM dunk Identification of the direct target genes of transcription factors could shed light on how healthy cells become malignant. Muhar et al. applied a modified version of a transcript-mapping method called SLAM-seq to identify the target genes of two transcriptional regulators of major interest in cancer research (see the Perspective by Sabò and Amati). The MYC oncoprotein selectively activates transcription of just a few genes, primarily those involved in basic cell metabolism. In contrast, BRD4, a bromodomain-containing protein that is being targeted for cancer therapy, activates transcription of many genes. Science , this issue p. 800 ; see also p. 713

Funder

H2020 European Research Council

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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