The Calicheamicin Gene Cluster and Its Iterative Type I Enediyne PKS-Reference-Cited by-同舟云学术

The Calicheamicin Gene Cluster and Its Iterative Type I Enediyne PKS

Published:2002-08-16 Issue:5584 Volume:297 Page:1173-1176
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Ahlert Joachim¹,Shepard Erica¹,Lomovskaya Natalia¹,Zazopoulos Emmanuel²,Staffa Alfredo²,Bachmann Brian O.²,Huang Kexue²,Fonstein Leonid¹,Czisny Anne¹,Whitwam Ross E.¹,Farnet Chris M.²,Thorson Jon S.¹

Affiliation:

1. Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA.

2. Ecopia Biosciences Inc., 7290 Frederick-Banting, Montreal, Quebec, Canada H4S 2A1.

Abstract

The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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