Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity-Reference-Cited by-同舟云学术

Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity

Published:2001-03-09 Issue:5510 Volume:291 Page:1965-1969
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Volchkov Viktor E.¹²,Volchkova Valentina A.¹,Mühlberger Elke¹,Kolesnikova Larissa V.¹,Weik Michael¹,Dolnik Olga¹,Klenk Hans-Dieter¹

Affiliation:

1. Institut für Virologie, Philipps-Universität, Robert-Koch-Strasse 17, 35037 Marburg, Germany.

2. University Claude Bernard Lyon-1, Filovirus Laboratory, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07, France.

Abstract

To study the mechanisms underlying the high pathogenicity of Ebola virus, we have established a system that allows the recovery of infectious virus from cloned cDNA and thus permits genetic manipulation. We created a mutant in which the editing site of the gene encoding envelope glycoprotein (GP) was eliminated. This mutant no longer expressed the nonstructural glycoprotein sGP. Synthesis of GP increased, but most of it accumulated in the endoplasmic reticulum as immature precursor. The mutant was significantly more cytotoxic than wild-type virus, indicating that cytotoxicity caused by GP is down-regulated by the virus through transcriptional RNA editing and expression of sGP.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference31 articles.

1. C. J. Peters A. Sanchez P. E. Rollin T. G. Ksiazek F. A. Murphy in Fields Virology B. N. Fields et al. Eds. (Raven Philadelphia ed. 3 1996) pp. 1161–1176.

2. H. Feldmann M. P. Kiley in Marburg and Ebola Viruses H.-D. Klenk Ed. (Springer-Verlag Berlin 1999) pp. 1–21.

3. GP mRNA of Ebola Virus Is Edited by the Ebola Virus Polymerase and by T7 and Vaccinia Virus Polymerases1

4. The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing.

5. A system for functional analysis of Ebola virus glycoprotein

Cited by 254 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Development of a Měnglà virus minigenome and comparison of its polymerase complexes with those of other filoviruses;Virologica Sinica;2024-06

2. Developments in Negative-Strand RNA Virus Reverse Genetics;Microorganisms;2024-03-11

3. Negative-Strand RNA Virus-Vectored Vaccines;Methods in Molecular Biology;2024

4. Reverse Genetics Systems for Filoviruses;Methods in Molecular Biology;2023-12-09

5. What Have We Learned by Resurrecting the 1918 Influenza Virus?;Annual Review of Virology;2023-09-29