TRPM4 Regulates Calcium Oscillations After T Cell Activation

Abstract

TRPM4 has recently been described as a calcium-activated nonselective (CAN) cation channel that mediates membrane depolarization. However, the functional importance of TRPM4 in the context of calcium (Ca 2+ ) signaling and its effect on cellular responses are not known. Here, the molecular inhibition of endogenous TRPM4 in T cells was shown to suppress TRPM4 currents, with a profound influence on receptor-mediated Ca 2+ mobilization. Agonist-mediated oscillations in intracellular Ca 2+ concentration ([Ca 2+ ] i ), which are driven by store-operated Ca 2+ influx, were transformed into a sustained elevation in [Ca 2+ ] i . This increase in Ca 2+ influx enhanced interleukin-2 production. Thus, TRPM4-mediated depolarization modulates Ca 2+ oscillations, with downstream effects on cytokine production in T lymphocytes.