A Cas9–guide RNA complex preorganized for target DNA recognition-Reference-Cited by-同舟云学术

A Cas9–guide RNA complex preorganized for target DNA recognition

Published:2015-06-26 Issue:6242 Volume:348 Page:1477-1481
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Jiang Fuguo¹,Zhou Kaihong²,Ma Linlin²,Gressel Saskia³,Doudna Jennifer A.¹²⁴⁵⁶

Affiliation:

1. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

2. Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.

3. Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany.

4. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA.

5. Department of Chemistry, University of California, Berkeley, CA 94720, USA.

6. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Abstract

An RNA seed poised to meet its target The CRISPR-Cas system in prokaryotes precisely identifies infecting parasitic DNAs and viruses and destroys them. The CRISPR-Cas system has been adapted for facile genome editing, heralding a new age in molecular biology. Jiang et al. show that the Cas9 nuclease adopts a distinct confirmation when it binds to the targeting guide RNA. The guide RNA then assumes a preordered shape. This RNA “seed region” is thus poised to initiate recognition of the DNA target sequence. Science , this issue p. 1477

Funder

NSF

Howard Hughes Medical Institute

Damon Runyon Cancer Research Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference41 articles.

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3. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity