Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry-Reference-Cited by-同舟云学术

Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry

Published:2018-11-16 Issue:6416 Volume:362 Page:829-834
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Chorev Dror S.¹^ORCID,Baker Lindsay A.²^ORCID,Wu Di¹,Beilsten-Edmands Victoria¹,Rouse Sarah L.³^ORCID,Zeev-Ben-Mordehai Tzviya²^ORCID,Jiko Chimari⁴,Samsudin Firdaus⁵^ORCID,Gerle Christoph⁶⁷^ORCID,Khalid Syma⁵,Stewart Alastair G.⁸⁹^ORCID,Matthews Stephen J.³^ORCID,Grünewald Kay²¹⁰^ORCID,Robinson Carol V.¹^ORCID

Affiliation:

1. Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, UK.

2. Division of Structural Biology, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.

3. Department of Life Sciences, Imperial College, London, South Kensington Campus, London SW7 2AZ, UK.

4. Institute for Integrated Radiation and Nuclear Science, Kyoto University, Kumatori, Japan.

5. School of Chemistry, University of Southampton, University Road, Southampton SO17 1BJ, UK.

6. Institute for Protein Research, Osaka University, Suita, Osaka, Japan.

7. Core Research for Evolutional Science and Technology, Japan and Science and Technology Agency, Kawaguchi, Japan.

8. Molecular, Structural and Computational Biology Division, Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia.

9. Faculty of Medicine, The University of New South Wales, Sydney, NSW, Australia.

10. Centre of Structural Systems Biology (CSSB), Notkestr. 85, D-22607, Heinrich-Pette Institute/University of Hamburg, Hamburg, Germany.

Abstract

Innovating to be nondisruptive Insights into the architecture and stoichiometry of membrane complexes have grown with advances in cryo–electron microscopy and native mass spectroscopy. However, most of these studies are not in the context of native membrane. Chorev et al. released intact membrane complexes directly from native lipid membrane vesicles into a mass spectrometer. They analyzed components of the Escherichia coli inner and outer membranes and the bovine mitochondrial inner membrane. For several identified complexes, they found a stoichiometry that differs from published results and, in some cases, confirmed interactions that could not be characterized structurally. Science , this issue p. 829

Funder

Wellcome Trust

BBSRC

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference63 articles.

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