Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors-Reference-Cited by-同舟云学术

Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors

Published:2017-04-21 Issue:6335 Volume:356 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Villani Alexandra-Chloé¹²^ORCID,Satija Rahul¹³⁴^ORCID,Reynolds Gary⁵^ORCID,Sarkizova Siranush¹,Shekhar Karthik¹,Fletcher James⁵^ORCID,Griesbeck Morgane⁶^ORCID,Butler Andrew³⁴^ORCID,Zheng Shiwei³⁴^ORCID,Lazo Suzan⁷^ORCID,Jardine Laura⁵,Dixon David⁵^ORCID,Stephenson Emily⁵^ORCID,Nilsson Emil⁸^ORCID,Grundberg Ida⁸^ORCID,McDonald David⁵^ORCID,Filby Andrew⁵,Li Weibo¹²,De Jager Philip L.¹⁹,Rozenblatt-Rosen Orit¹,Lane Andrew A.¹⁷,Haniffa Muzlifah⁵¹⁰^ORCID,Regev Aviv¹¹¹¹²^ORCID,Hacohen Nir¹²^ORCID

Affiliation:

1. Broad Institute of MIT and Harvard, Cambridge, MA, USA.

2. Center for Cancer Research, Massachusetts General Hospital, Department of Medicine, Boston, MA, USA.

3. New York Genome Center, New York University Center for Genomics and Systems Biology, New York, NY, USA.

4. New York University Center for Genomics and Systems Biology, New York, NY, USA.

5. Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.

6. Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard University, Cambridge, MA, USA.

7. Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA.

8. Olink Proteomics, Watertown, MA, USA.

9. Program in Translational NeuroPsychiatric Genomics, Department of Neurology, Brigham and Women’s Hospital and Harvard Medical School.

10. Department of Dermatology, Royal Victoria Infirmary, Newcastle Hospitals NHS Foundation Trust, UK.

11. Department of Biology and Koch Institute, Massachusetts Institute of Technology, Cambridge, MA, USA.

12. Howard Hughes Medical Institute, Chevy Chase, MD, USA.

Abstract

What's in a drop of blood? Blood contains many types of cells, including many immune system components. Immune cells used to be characterized by marker-based assays, but now classification relies on the genes that cells express. Villani et al. used deep sequencing at the single-cell level and unbiased clustering to define six dendritic cell and four monocyte populations. This refined analysis has identified, among others, a previously unknown dendritic cell population that potently activates T cells. Further cell culture revealed possible differentiation progenitors within the different cell populations. Science , this issue p. eaah4573

Funder

National Human Genome Research Institute Centers of Excellence in Genomics Science

Manton Foundation

Howard Hughes Medical Institute

NIH BRAIN

Klarman Family Foundation

NIH Director’s New Innovator Award

American Society of Hematology Scholar

Wellcome Trust

Publisher

American Association for the Advancement of Science (AAAS)

Subject