Design and synthesis of a minimal bacterial genome-Reference-Cited by-同舟云学术

Design and synthesis of a minimal bacterial genome

Published:2016-03-25 Issue:6280 Volume:351 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Hutchison Clyde A.¹,Chuang Ray-Yuan¹,Noskov Vladimir N.¹,Assad-Garcia Nacyra¹,Deerinck Thomas J.²,Ellisman Mark H.²,Gill John³,Kannan Krishna³,Karas Bogumil J.¹,Ma Li¹,Pelletier James F.⁴,Qi Zhi-Qing³,Richter R. Alexander¹,Strychalski Elizabeth A.⁴,Sun Lijie¹,Suzuki Yo¹,Tsvetanova Billyana³,Wise Kim S.¹,Smith Hamilton O.¹³,Glass John I.¹,Merryman Chuck¹,Gibson Daniel G.¹³,Venter J. Craig¹³

Affiliation:

1. J. Craig Venter Institute, La Jolla, CA 92037, USA.

2. National Center for Microscopy and Imaging Research, University of California–San Diego, La Jolla, CA 92037, USA.

3. Synthetic Genomics, La Jolla, CA 92037, USA.

4. National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.

Abstract

Designing and building a minimal genome A goal in biology is to understand the molecular and biological function of every gene in a cell. One way to approach this is to build a minimal genome that includes only the genes essential for life. In 2010, a 1079-kb genome based on the genome of Mycoplasma mycoides (JCV-syn1.0) was chemically synthesized and supported cell growth when transplanted into cytoplasm. Hutchison III et al. used a design, build, and test cycle to reduce this genome to 531 kb (473 genes). The resulting JCV-syn3.0 retains genes involved in key processes such as transcription and translation, but also contains 149 genes of unknown function. Science , this issue p. 10.1126/science.aad6253

Funder

NIH

National Institute of General Medical Sciences

Fannie and John Hertz Graduate Fellowship

National Institute of Standards and Technology

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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