Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans-Reference-Cited by-同舟云学术

Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans

Published:1999-09-03 Issue:5433 Volume:285 Page:1558-1562
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Lin Jieyi¹,Qi Rong¹,Aston Christopher¹,Jing Junping¹,Anantharaman Thomas S.²,Mishra Bud²,White Owen³,Daly Michael J.⁴,Minton Kenneth W.⁴,Venter J. Craig⁵,Schwartz David C.¹²

Affiliation:

1. W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY 10003, USA.

2. Courant Institute of Mathematical Sciences, Department of Computer Science, New York University, 251 Mercer Street, New York, NY 10012, USA.

3. The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.

4. Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

5. Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.

Abstract

A whole-genome restriction map of Deinococcus radiodurans , a radiation-resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis. Very large, randomly sheared, genomic DNA fragments were used to construct maps from individual DNA molecules that were assembled into two circular overlapping maps (2.6 and 0.415 megabases), without gaps. A third smaller chromosome (176 kilobases) was identified and characterized. Aberrant nonlinear DNA structures that may define chromosome structure and organization, as well as intermediates in DNA repair, were directly visualized by optical mapping techniques after γ irradiation.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference50 articles.

1. Schwartz D. C., Cantor C. R., Cell 37, 67 (1984);

2. A Physical Map of the Escherichia coli K12 Genome

3. Kohara Y., Akiyama K., Isono K., Cell 50, 495 (1987).

4. Link A. J., Olson M. V., Genetics 127, 681 (1991);

5. Riles L. et al. 134 81 (1993).

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