Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization

Author:

Yildiz Ahmet123,Forkey Joseph N.123,McKinney Sean A.123,Ha Taekjip123,Goldman Yale E.123,Selvin Paul R.123

Affiliation:

1. Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, IL 61801, USA.

2. Physics Department, University of Illinois, Urbana-Champaign, IL 61801, USA.

3. University of Pennsylvania, Pennsylvania Muscle Institute, Philadelphia, PA 19104, USA.

Abstract

Myosin V is a dimeric molecular motor that moves processively on actin, with the center of mass moving ∼37 nanometers for each adenosine triphosphate hydrolyzed. We have labeled myosin V with a single fluorophore at different positions in the light-chain domain and measured the step size with a standard deviation of <1.5 nanometers, with 0.5-second temporal resolution, and observation times of minutes. The step size alternates between 37 + 2x nm and 37 – 2x, where x is the distance along the direction of motion between the dye and the midpoint between the two heads. These results strongly support a hand-over-hand model of motility, not an inchworm model.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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