VirB/D4-Dependent Protein Translocation from Agrobacterium into Plant Cells-Reference-Cited by-同舟云学术

VirB/D4-Dependent Protein Translocation from Agrobacterium into Plant Cells

Published:2000-11-03 Issue:5493 Volume:290 Page:979-982
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Vergunst Annette C.¹,Schrammeijer Barbara¹,den Dulk-Ras Amke¹,de Vlaam Clementine M. T.¹,Regensburg-Tuı̈nk Tonny J. G.¹,Hooykaas Paul J. J.¹

Affiliation:

1. Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands.

Abstract

The Agrobacterium VirB/D4 transport system mediates the transfer of a nucleoprotein T complex into plant cells, leading to crown gall disease. In addition, several Virulence proteins must somehow be transported to fulfill a function in planta. Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells. Transport of the proteins was monitored by a Cre-mediated in planta recombination event resulting in a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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