PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux-Reference-Cited by-同舟云学术

PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux

Published:2006-05-12 Issue:5775 Volume:312 Page:914-918
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Petrášek Jan¹²³⁴⁵,Mravec Jozef¹²³⁴⁵,Bouchard Rodolphe¹²³⁴⁵,Blakeslee Joshua J.¹²³⁴⁵,Abas Melinda¹²³⁴⁵,Seifertová Daniela¹²³⁴⁵,Wiśniewska Justyna¹²³⁴⁵,Tadele Zerihun¹²³⁴⁵,Kubeš Martin¹²³⁴⁵,Čovanová Milada¹²³⁴⁵,Dhonukshe Pankaj¹²³⁴⁵,Skůpa Petr¹²³⁴⁵,Benková Eva¹²³⁴⁵,Perry Lucie¹²³⁴⁵,Křeček Pavel¹²³⁴⁵,Lee Ok Ran¹²³⁴⁵,Fink Gerald R.¹²³⁴⁵,Geisler Markus¹²³⁴⁵,Murphy Angus S.¹²³⁴⁵,Luschnig Christian¹²³⁴⁵,Zažímalová Eva¹²³⁴⁵,Friml Jiří¹²³⁴⁵

Affiliation:

1. Institute of Experimental Botany, the Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic.

2. Department of Plant Physiology, Faculty of Science, Charles University, 128 44 Prague 2, Czech Republic.

3. Center for Plant Molecular Biology (ZMBP), University Tübingen, D-72076 Tübingen, Germany.

4. Zurich-Basel Plant Science Center, University of Zurich, Institute of Plant Biology, CH 8007 Zurich, Switzerland.

5. Department of Horticulture, Purdue University, West Lafayette, IN 47907, USA.

Abstract

Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport–related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference24 articles.

1. Auxin transport — shaping the plant

2. Auxin: Regulation, Action, and Interaction

3. Plant Development: Auxin in Loops

4. Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis

5. AtPIN4 Mediates Sink-Driven Auxin Gradients and Root Patterning in Arabidopsis

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