Affiliation:
1. Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA.
Abstract
The Power Behind P450
Drugs, toxins and a range of metabolic substrates are detoxified in the liver by family of iron-containing enzymes called cytochrome P450. The iron component transfers oxygen to compounds that are often highly resistant to chemical reaction, but we know very little about the mechanism of this vital detoxification process.
Rittle and Green
(p.
933
; see the Perspective by
Sligar
) have managed to capture the P450 reaction intermediate by freezing a solution of the enzyme as it reacts with an oxidant. Spectroscopic techniques and kinetic studies then revealed an iron(IV)oxo intermediate that passes its oxygen along to the substrate with remarkable speed.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
1165 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献