Polarization-Enhanced NMR Spectroscopy of Biomolecules in Frozen Solution-Reference-Cited by-同舟云学术

Polarization-Enhanced NMR Spectroscopy of Biomolecules in Frozen Solution

Published:1997-05-09 Issue:5314 Volume:276 Page:930-932
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Hall Dennis A.¹²³,Maus Douglas C.¹²³,Gerfen Gary J.¹²³,Inati Souheil J.¹²³,Becerra Lino R.¹²³,Dahlquist Frederick W.¹²³,Griffin Robert G.¹²³

Affiliation:

1. D. A. Hall, D. C. Maus, G. J. Gerfen, L. R. Becerra, R. G. Griffin, Francis Bitter Magnet Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

2. S. J. Inati, Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

3. F. W. Dahlquist, Department of Chemistry, University of Oregon, Eugene, OR 97403, USA.

Abstract

Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference38 articles.

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