Bimolecular Exon Ligation by the Human Spliceosome

Author:

Anderson Karin1,Moore Melissa J.1

Affiliation:

1. W. M. Keck Institute for Cellular Visualization, Department of Biochemistry, Brandeis University, Waltham, MA 02254, USA.

Abstract

Intron excision is an essential step in eukaryotic gene expression, but the molecular mechanisms by which the spliceosome accurately identifies splice sites in nuclear precursors to messenger RNAs (pre-mRNAs) are not well understood. A bimolecular assay for the second step of splicing has now revealed that exon ligation by the human spliceosome does not require covalent attachment of a 3′ splice site to the branch site. Furthermore, accurate definition of the 3′ splice site in this system is independent of either a covalently attached polypyrimidine tract or specific 3′ exon sequences. Rather, in this system 3′ splice site selection apparently occurs with a 5′ → 3′ directionality.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference60 articles.

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2. Nilsen T. W., Cell 78, 1 (1994);

3. ; A. Krämer in Pre-mRNA Processing A. I. Lamond Ed. (Landes Austin TX 1995) pp. 35–64.

4. Lariat RNA's as Intermediates and Products in the Splicing of Messenger RNA precursors

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