Menin “reads” H3K79me2 mark in a nucleosomal context

Author:

Lin Jianwei1ORCID,Wu Yiping1ORCID,Tian Gaofei1ORCID,Yu Daqi2ORCID,Yang Eunjeong34,Lam Wai Hei5ORCID,Liu Zheng1,Jing Yihang6ORCID,Dang Shangyu2ORCID,Bao Xiucong3ORCID,Wong Jason Wing Hon34ORCID,Zhai Yuanliang5ORCID,Li Xiang David16ORCID

Affiliation:

1. Department of Chemistry, University of Hong Kong, Hong Kong SAR, China.

2. Division of Life Science, Hong Kong University of Science and Technology, Hong Kong SAR, China.

3. School of Biomedical Sciences, University of Hong Kong, Hong Kong SAR, China.

4. Centre for Oncology and Immunology, Hong Kong Science Park, Hong Kong SAR, China.

5. School of Biological Sciences, University of Hong Kong, Hong Kong SAR, China.

6. Greater Bay Biomedical InnoCenter, Shenzhen Bay Laboratory, Shenzhen, China.

Abstract

Methylation of histone H3 lysine-79 (H3K79) is an epigenetic mark for gene regulation in development, cellular differentiation, and disease progression. However, how this histone mark is translated into downstream effects remains poorly understood owing to a lack of knowledge about its readers. We developed a nucleosome-based photoaffinity probe to capture proteins that recognize H3K79 dimethylation (H3K79me2) in a nucleosomal context. In combination with a quantitative proteomics approach, this probe identified menin as a H3K79me2 reader. A cryo–electron microscopy structure of menin bound to an H3K79me2 nucleosome revealed that menin engages with the nucleosome using its fingers and palm domains and recognizes the methylation mark through a π-cation interaction. In cells, menin is selectively associated with H3K79me2 on chromatin, particularly in gene bodies.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Cited by 28 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3