Field-deployable viral diagnostics using CRISPR-Cas13-Reference-Cited by-同舟云学术

Field-deployable viral diagnostics using CRISPR-Cas13

Published:2018-04-27 Issue:6387 Volume:360 Page:444-448
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Myhrvold Cameron¹²^ORCID,Freije Catherine A.¹²³^ORCID,Gootenberg Jonathan S.¹⁴⁵⁶⁷^ORCID,Abudayyeh Omar O.¹⁵⁶⁷⁸^ORCID,Metsky Hayden C.¹⁹^ORCID,Durbin Ann F.³¹⁰^ORCID,Kellner Max J.¹^ORCID,Tan Amanda L.¹¹^ORCID,Paul Lauren M.¹¹^ORCID,Parham Leda A.¹²^ORCID,Garcia Kimberly F.¹²^ORCID,Barnes Kayla G.¹²¹³,Chak Bridget¹²^ORCID,Mondini Adriano¹⁴^ORCID,Nogueira Mauricio L.¹⁵^ORCID,Isern Sharon¹¹,Michael Scott F.¹¹,Lorenzana Ivette¹²,Yozwiak Nathan L.¹²^ORCID,MacInnis Bronwyn L.¹¹³,Bosch Irene¹⁰¹⁶^ORCID,Gehrke Lee³¹⁰¹⁷^ORCID,Zhang Feng¹⁵⁶⁷^ORCID,Sabeti Pardis C.¹²³¹³¹⁸^ORCID

Affiliation:

1. Broad Institute of the Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.

2. Center for Systems Biology, Department of Organismal and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.

3. Ph.D. Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA 02115, USA.

4. Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.

5. McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA.

6. Department of Brain and Cognitive Science, MIT, Cambridge, MA 02139, USA.

7. Department of Biological Engineering, MIT, Cambridge, MA 02139, USA.

8. Department of Health Sciences and Technology, MIT, Cambridge, MA 02139, USA.

9. Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA.

10. Institute for Medical Engineering and Science, MIT, Cambridge, MA 02139, USA.

11. Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, FL 33965, USA.

12. Centro de Investigaciones Genética, Instituto de Investigacion en Microbiologia, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras.

13. Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, MA 02115, USA.

14. Araraquara Laboratory of Public Health, School of Pharmaceutical Sciences, São Paulo State University, São Paulo, Brazil.

15. Laboratorio de Pesquisas em Virologia, Faculdade de Medicina de Sao Jose do Rio Preto, São Paulo, Brazil.

16. Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.

17. Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA.

18. Howard Hughes Medical Institute (HHMI), Chevy Chase, MD 20815, USA.

Abstract

Taking CRISPR technology further CRISPR techniques are allowing the development of technologies for nucleic acid detection (see the Perspective by Chertow). Taking advantages of the distinctive enzymatic properties of CRISPR enzymes, Gootenberg et al. developed an improved nucleic acid detection technology for multiplexed quantitative and highly sensitive detection, combined with lateral flow for visual readout. Myhrvold et al. added a sample preparation protocol to create a field-deployable viral diagnostic platform for rapid detection of specific strains of pathogens in clinical samples. Cas12a (also known as Cpf1), a type V CRISPR protein, cleaves double-stranded DNA and has been adapted for genome editing. Chen et al. discovered that Cas12a also processes single-stranded DNA threading activity. A technology platform based on this activity detected human papillomavirus in patient samples with high sensitivity. Science , this issue p. 439 , p. 444 , p. 436 ; see also p. 381

Funder

Howard Hughes Medical Institute

NIH Office of the Director

Defense Sciences Office, DARPA

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference31 articles.

1. One-step RT-PCR for detection of Zika virus

2. Persistence of Zika Virus in Body Fluids — Final Report

3. Zika virus evolution and spread in the Americas