Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails-Reference-Cited by-同舟云学术

Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails

Published:2019-01-18 Issue:6424 Volume:363 Page:285-288
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Schmidt-Cernohorska M.¹^ORCID,Zhernov I.²³^ORCID,Steib E.¹^ORCID,Le Guennec M.¹,Achek R.⁴,Borgers S.¹,Demurtas D.⁵,Mouawad L.⁴^ORCID,Lansky Z.²^ORCID,Hamel V.¹^ORCID,Guichard P.¹^ORCID

Affiliation:

1. Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland.

2. Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic.

3. Faculty of Mathematics and Physics, Charles University in Prague, Prague, Czech Republic.

4. Institut Curie, PSL Research University, CNRS UMR 9187 – INSERM U1196, Paris-Saclay University, F-91405 Orsay, France.

5. Interdisciplinary Centre for Electron Microscopy, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.

Abstract

Assembly of the ciliary microtubule doublet The cilium is a conserved organelle that is crucial for motility as well as for sensing the extracellular environment. Its core structure is characterized by nine microtubule doublets (MTDs). The mechanisms of MTD assembly are unclear. Schmidt-Cernohorska et al. developed an assay to reconstitute MTD assembly in vitro. Tubulin carboxyl-terminal tails played a critical inhibitory role in MTD formation. Molecular dynamics revealed that carboxyl-terminal tails of the A11 microtubule protofilament regulated MTD initiation. Furthermore, live-cell imaging showed an unexpected bidirectional isotropic elongation of the MTD. Science , this issue p. 285

Funder

H2020 European Research Council

Swiss National Science Foundation

Czech Science Foundation

ERDF

GAUK

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference32 articles.

1. The Cilium: Cellular Antenna and Central Processing Unit