Semisynthetic sensor proteins enable metabolic assays at the point of care-Reference-Cited by-同舟云学术

Semisynthetic sensor proteins enable metabolic assays at the point of care

Published:2018-09-14 Issue:6407 Volume:361 Page:1122-1126
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Yu Qiuliyang¹²^ORCID,Xue Lin¹^ORCID,Hiblot Julien¹^ORCID,Griss Rudolf²,Fabritz Sebastian¹^ORCID,Roux Clothilde³,Binz Pierre-Alain³^ORCID,Haas Dorothea⁴,Okun Jürgen G.⁴,Johnsson Kai¹²

Affiliation:

1. Department of Chemical Biology, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany.

2. École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.

3. University Hospital of Lausanne, Service of Biomedicine, Clinical Chemistry Laboratory, 1011 Lausanne, Switzerland.

4. University Children’s Hospital Heidelberg, Center for Metabolic Diseases, Metabolic Laboratory, 69120 Heidelberg, Germany.

Abstract

A protein designed to sense metabolites Many diseases cause characteristic changes in blood metabolites. Yu et al. describe a paper-based assay in which a chosen metabolite can be oxidized to generate reduced nicotinamide adenine dinucleotide phosphate (NADPH). Color changes in a designed NADPH sensor protein are then quantified by a digital camera. The sensor system successfully generated point-of-care measurements of phenylalanine, glucose, and glutamate. Concentrations of phenylalanine in the blood of phenylketonuria patients were analyzed within minutes with only half a microliter of blood. Science , this issue p. 1122

Funder

École Polytechnique Fédérale de Lausanne

Swiss National Science Foundation

Max-Planck-Gesellschaft

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference37 articles.

1. Phenylketonuria

2. Progression of ischaemic stroke and excitotoxic aminoacids

3. Classical galactosaemia revisited