Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences-Reference-Cited by-同舟云学术

Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences

Published:2009-05-08 Issue:5928 Volume:324 Page:797-801
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Yu Junying¹²³,Hu Kejin³,Smuga-Otto Kim¹²³,Tian Shulan¹²,Stewart Ron¹²,Slukvin Igor I.³⁴,Thomson James A.¹²³⁵

Affiliation:

1. Morgridge Institute for Research, Madison, WI 53707–7365, USA.

2. Genome Center of Wisconsin, Madison, WI 53706–1580, USA.

3. Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715–1299, USA.

4. Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.

5. Department of Anatomy, University of Wisconsin-Madison, Madison, WI 53706–1509, USA.

Abstract

Designer Stem Cells Despite their promise for use as disease models and in regenerative medicine, the generation of human-induced pluripotent stem (iPS) cells has been hindered by the integration of vector and transgenes in the host cell genome. Recent studies using the Cre/LoxP recombination strategy and the piggyBac transposon approach have approached this objective. However, Yu et al. (p. 797 , published online 26 March) now show the derivation of human iPS cells from postnatal foreskin fibroblasts using the nonintegrating oriP/EBNA1-based episomal vectors. The resultant iPS cells show characteristics of human embryonic stem cells and are free of vector and transgenes.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference22 articles.

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4. Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors

5. Generation of germline-competent induced pluripotent stem cells

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