Affiliation:
1. The J. Craig Venter Institute, Rockville, MD 20850, USA.
Abstract
As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from
Mycoplasma mycoides
large colony (LC), virtually free of protein, was transplanted into
Mycoplasma capricolum
cells by polyethylene glycol–mediated transformation. Cells selected for tetracycline resistance, carried by the
M. mycoides
LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the
M. mycoides
LC donor strain as judged by several criteria.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
351 articles.
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