Affiliation:
1. VA Greater Los Angeles Healthcare System and Departments of Physiology and Medicine, University of California, Los Angeles, CA 90073, USA.
Abstract
Acidic media trigger cytoplasmic urease activity of the unique human gastric pathogen
Helicobacter pylori.
Deletion of
ureI
prevents this activation of cytoplasmic urease that is essential for bacterial acid resistance. UreI is an inner membrane protein with six transmembrane segments as shown by in vitro transcription/translation and membrane separation. Expression of UreI in
Xenopus
oocytes results in acid-stimulated urea uptake, with a pH profile similar to activation of cytoplasmic urease. Mutation of periplasmic histidine 123 abolishes stimulation. UreI-mediated transport is urea specific, passive, nonsaturable, nonelectrogenic, and temperature independent. UreI functions as a H
+
-gated urea channel regulating cytoplasmic urease that is essential for gastric survival and colonization.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
413 articles.
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