Structures and operating principles of the replisome-Reference-Cited by-同舟云学术

Structures and operating principles of the replisome

Published:2019-02-22 Issue:6429 Volume:363 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Gao Yang¹^ORCID,Cui Yanxiang²^ORCID,Fox Tara³⁴^ORCID,Lin Shiqiang¹^ORCID,Wang Huaibin¹^ORCID,de Val Natalia³⁴,Zhou Z. Hong²^ORCID,Yang Wei¹^ORCID

Affiliation:

1. Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

2. The California NanoSystems Institute, UCLA, Los Angeles, CA 90095, USA.

3. Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

4. Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, MD 21701, USA.

Abstract

Structures of the simplest replisome The DNA replisome performs concerted parental-strand separation and DNA synthesis on both strands. Gao et al. report the cryo–electron microscopy structures of the minimum set of bacteriophage T7 proteins that can carry out leading- and lagging-strand synthesis at the replication fork (see the Perspective by Li and O'Donnell). Three key enzymes involved in DNA replication—DNA polymerase, helicase, and primase—were visualized in complex with substrate DNA, demonstrating their highly dynamic organizations on both strands. Comparison of prokaryotic and eukaryotic replisomes reveals evolutionarily conserved operating principles and provides a structural basis for understanding coordination among DNA replication, recombination, and repair. Science , this issue p. eaav7003 ; see also p. 814

Funder

National Science Foundation

National Institutes of Health

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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