Barcoded viral tracing of single-cell interactions in central nervous system inflammation-Reference-Cited by-同舟云学术

Barcoded viral tracing of single-cell interactions in central nervous system inflammation

Published:2021-04-23 Issue:6540 Volume:372 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Clark Iain C.¹²^ORCID,Gutiérrez-Vázquez Cristina¹^ORCID,Wheeler Michael A.¹³^ORCID,Li Zhaorong¹³^ORCID,Rothhammer Veit¹⁴,Linnerbauer Mathias¹⁴^ORCID,Sanmarco Liliana M.¹^ORCID,Guo Lydia¹^ORCID,Blain Manon⁵,Zandee Stephanie E. J.⁶^ORCID,Chao Chun-Cheih¹^ORCID,Batterman Katelyn V.⁷^ORCID,Schwabenland Marius⁸^ORCID,Lotfy Peter¹³^ORCID,Tejeda-Velarde Amalia¹^ORCID,Hewson Patrick¹^ORCID,Manganeli Polonio Carolina¹^ORCID,Shultis Michael W.¹,Salem Yasmin¹^ORCID,Tjon Emily C.¹,Fonseca-Castro Pedro H.¹^ORCID,Borucki Davis M.¹^ORCID,Alves de Lima Kalil¹^ORCID,Plasencia Agustin¹^ORCID,Abate Adam R.⁹¹⁰^ORCID,Rosene Douglas L.⁷^ORCID,Hodgetts Kevin J.¹^ORCID,Prinz Marco⁸¹¹¹²^ORCID,Antel Jack P.⁵^ORCID,Prat Alexandre⁶^ORCID,Quintana Francisco J.¹³^ORCID

Affiliation:

1. Ann Romney Center for Neurologic Diseases, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA.

2. Department of Bioengineering, University of California, Berkeley, California Institute for Quantitative Biosciences, Berkeley, CA 94720, USA.

3. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

4. Department of Neurology, University Hospital Erlangen, Friedrich–Alexander University Erlangen–Nürnberg, 91054 Erlangen, Germany.

5. Neuroimmunology Unit, Montreal Neurological Institute, Department of Neurology and Neurosurgery, McGill University, Montreal, QC H3A 2B4, Canada.

6. Neuroimmunology Research Laboratory, Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montreal, QC H2X 0A9, Canada.

7. Department of Anatomy and Neurobiology, Boston University School of Medicine, Boston, MA 02118, USA.

8. Institute of Neuropathology, University of Freiburg, D-79106 Freiburg, Germany.

9. Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California Institute for Quantitative Biosciences, San Francisco, CA 94158, USA.

10. Chan Zuckerberg Biohub, San Francisco, CA, USA.

11. Signaling Research Centres BIOSS and CIBSS, University of Freiburg, D-79106 Freiburg, Germany.

12. Center for Basics in NeuroModulation (NeuroModulBasics), Faculty of Medicine, University of Freiburg, D-79106 Freiburg, Germany.

Abstract

Single-cell analysis of CNS interactions Despite their importance in the physiology and pathology of the central nervous system (CNS), few methods are available for the unbiased, systematic investigation of cell-to-cell interactions at single-cell resolution. Clark et al. developed RABID-seq, a method that combines barcoded viral tracing with single-cell RNA sequencing (see the Perspective by Silvin and Ginhoux). RABID-seq identified the axon guidance molecules Sema4D-PlexinB2 and EphrinB3-EphB3 as mediators of microglia-astrocyte interactions that promote CNS pathology in experimental autoimmune encephalomyelitis and, potentially, multiple sclerosis. These studies also identified candidate therapeutic molecules for the modulation of microglia-astrocyte interactions in multiple sclerosis. Science , this issue p. eabf1230 ; see also p. 342

Funder

National Institutes of Health

American Cancer Society

National Multiple Sclerosis Society

ASCRS Research Foundation

Fonds de Recherche du Québec - Santé

Deutsche Forschungsgemeinschaft

São Paulo Research Foundation

National Cancer Institute, Cairo University

Ministry of Science and Technology, Government of the People’s Republic of Bangladesh

This

Alfonso Mart Escudero Foundation