Affiliation:
1. Laboratory of Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Abstract
In contrast to current models, fluorescence resonance energy transfer measurements using a single-cell imaging assay with fluorescent forms of PER and TIM showed that these proteins bind rapidly and persist in the cytoplasm while gradually accumulating in discrete foci. After ∼6 hours, complexes abruptly dissociated, as PER and TIM independently moved to the nucleus in a narrow time frame. The
per
L
mutation delayed nuclear accumulation in vivo and in our cultured cell system, but without affecting rates of PER/TIM assembly or dissociation. This finding points to a previously unrecognized form of temporal regulation that underlies the periodicity of the circadian clock.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
151 articles.
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