Direct Coupling Between Meiotic DNA Replication and Recombination Initiation-Reference-Cited by-同舟云学术

Direct Coupling Between Meiotic DNA Replication and Recombination Initiation

Published:2000-10-27 Issue:5492 Volume:290 Page:806-809
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Borde Valérie¹,Goldman Alastair S. H.²,Lichten Michael¹

Affiliation:

1. Laboratory of Biochemistry, Division of Basic Science, National Cancer Institute, Bethesda, MD 20892–4255, USA.

2. Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK.

Abstract

During meiosis in Saccharomyces cerevisiae , DNA replication occurs 1.5 to 2 hours before recombination initiates by DNA double-strand break formation. We show that replication and recombination initiation are directly linked. Blocking meiotic replication prevented double-strand break formation in a replication-checkpoint–independent manner, and delaying replication of a chromosome segment specifically delayed break formation in that segment. Consequently, the time between replication and break formation was held constant in all regions. We suggest that double-strand break formation occurs as part of a process initiated by DNA replication, which thus determines when meiotic recombination initiates on a regional rather than a cell-wide basis.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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