Mechanism of Ammonia Transport by Amt/MEP/Rh: Structure of AmtB at 1.35 Å-Reference-Cited by-同舟云学术

Mechanism of Ammonia Transport by Amt/MEP/Rh: Structure of AmtB at 1.35 Å

Published:2004-09-10 Issue:5690 Volume:305 Page:1587-1594
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Khademi Shahram¹,O'Connell Joseph¹,Remis Jonathan¹,Robles-Colmenares Yaneth¹,Miercke Larry J. W.¹,Stroud Robert M.¹

Affiliation:

1. Department of Biochemistry and Biophysics, S412C Genentech Hall, University of California–San Francisco, 600 16th Street, San Francisco, CA 94143–2240, USA.

Abstract

The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH 4 + /NH 3 , a binding site for NH 4 + , and a 20 angstrom–long hydrophobic channel that lowers the NH 4 + p K a to below 6 and conducts NH 3 . Favorable interactions for NH 3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH 3 .

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference38 articles.

1. Mutant strains (nit) of Salmonella typhimurium with a pleiotropic defect in nitrogen metabolism

2. Ammonium transport in the kidney.

3. Phylogenetic characterization of novel transport protein families revealed by genome analyses

4. Membrane topology of the Mep/Amt family of ammonium transporters

5. Cloning and expression of the MEP1 gene encoding an ammonium transporter in Saccharomyces cerevisiae.

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