Design, synthesis, and testing toward a 57-codon genome-Reference-Cited by-同舟云学术

Design, synthesis, and testing toward a 57-codon genome

Published:2016-08-19 Issue:6301 Volume:353 Page:819-822
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Ostrov Nili¹,Landon Matthieu¹²³,Guell Marc¹⁴,Kuznetsov Gleb¹⁵,Teramoto Jun¹⁶,Cervantes Natalie¹,Zhou Minerva⁷,Singh Kerry⁷,Napolitano Michael G.¹⁸,Moosburner Mark¹,Shrock Ellen¹,Pruitt Benjamin W.⁴,Conway Nicholas⁴,Goodman Daniel B.¹⁴,Gardner Cameron L.¹,Tyree Gary¹,Gonzales Alexandra¹,Wanner Barry L.¹⁹,Norville Julie E.¹,Lajoie Marc J.¹,Church George M.¹⁴

Affiliation:

1. Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

2. Program in Systems Biology, Harvard University, Cambridge, MA 02138, USA.

3. Ecole des Mines de Paris, Mines Paristech, Paris 75272, France.

4. Wyss Institute for Biologically Inspired Engineering, Boston, MA 02115, USA.

5. Program in Biophysics, Harvard University, Boston, MA 02115, USA.

6. Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.

7. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

8. Program in Biological and Biomedical Sciences, Harvard University, Cambridge, MA 02138, USA.

9. Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA.

Abstract

Recoding and repurposing genetic codons By recoding bacterial genomes, it is possible to create organisms that can potentially synthesize products not commonly found in nature. By systematic replacement of seven codons with synonymous alternatives for all protein-coding genes, Ostrov et al. recoded the Escherichia coli genome. The number of codons in the E. coli genetic code was reduced from 64 to 57 by removing instances of the UAG stop codon and excising two arginine codons, two leucine codons, and two serine codons. Over 90% functionality was successfully retained. In 10 cases, reconstructed bacteria were not viable, but these few failures offered interesting insights into genome-design challenges and what is needed for a viable genome. Science , this issue p. 819

Funder

U.S Department of Energy

Defense Advanced Research Projects Agency

NSF

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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