Type IV Pili, Transient Bacterial Aggregates, and Virulence of Enteropathogenic
Escherichia coli
Author:
Bieber David123, Ramer Sandra W.123, Wu Cheng-Yen123, Murray William J.123, Tobe Toru123, Fernandez Rosemary123, Schoolnik Gary K.123
Affiliation:
1. D. Bieber, S. W. Ramer, C.-Y. Wu, R. Fernandez, G. K. Schoolnik, Department of Medicine, Division of Infectious Diseases and Geographical Medicine, and Department of Microbiology and Immunology, Stanford Program for Vaccine Research, Stanford University Medical Center, Stanford, CA 94305 USA. 2. W. J. Murray, Department of Biological Sciences, San Jose State University, San Jose, CA 95912 USA. 3. T. Tobe, Department of Bacteriology, Institute of Medicine, University of Tokyo, Tokyo, Japan.
Abstract
Type IV bundle-forming pili of enteropathogenic
Escherichia coli
are required for the localized adherence and autoaggregation phenotypes. Whether these pili are also required for virulence was tested in volunteers by inactivating
bfpA
or
bfpT
(
perA
) encoding, respectively, the pilus subunit and the
bfp
operon transcriptional activator. Both mutants caused significantly less diarrhea. Mutation of the
bfpF
nucleotide-binding domain caused increased piliation, enhanced localized adherence, and abolished the twitching motility–dispersal phase of the autoaggregation phenotype. The
bfpF
mutant colonized the human intestine but was about 200-fold less virulent. Thus, BfpF is required for dispersal from the bacterial aggregate and for full virulence.
Publisher
American Association for the Advancement of Science (AAAS)
Subject
Multidisciplinary
Reference32 articles.
1. P. A. Manning and T. F. Meyer Eds. Gene 192 1 (1997). 2. Herrington D. A., et al., J. Exp. Med.168, 1487 (1988); J. Swanson and J. M. Koomey, inMobile DNA, D. E. Berg and M. M. Howe, Eds. (American Society for Microbiology, Washington, DC, 1989), pp. 743–761. 3. Henrichsen J., Annu. Rev. Microbiol.37, 81 (1983). 4. M. S. Donnenberg in Infections of the Gastrointestinal Tract M. J. Blaser P. D. Smith J. I. Ravdin H. B. Greenberg R. L. Guerrant Eds. (Raven Press New York 1995) pp. 709–726. 5. M. M. Baldini, J. B. Kaper, M. Levine, D. Candy, H. Moon, J. Pediatr. Gastroenterol. Nutr. 2, 534 (1983); M. M. Baldini, J. P. Nataro, J. B. Kaper, Infect. Immun. 52, 334 (1986); A. Cravioto, R. Gross, S. Scotland, B. Rowe, Curr. Microbiol. 3, 95 (1979); I. C. Scaletsky, M. Silva, L. Trabulsi, Infect. Immun. 45, 534 (1984); Nataro J. P., Maher K., Mackie P., Kaper J., ibid 55, 2370 (1987); J. P. Nataro, I. Scaletsky, J. Kaper, M. Levine, L. Trabulsi, ibid. 48, 378 (1985).
Cited by
413 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|