Type IV Pili, Transient Bacterial Aggregates, and Virulence of Enteropathogenic Escherichia coli-Reference-Cited by-同舟云学术

Type IV Pili, Transient Bacterial Aggregates, and Virulence of Enteropathogenic Escherichia coli

Published:1998-06-26 Issue:5372 Volume:280 Page:2114-2118
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Bieber David¹²³,Ramer Sandra W.¹²³,Wu Cheng-Yen¹²³,Murray William J.¹²³,Tobe Toru¹²³,Fernandez Rosemary¹²³,Schoolnik Gary K.¹²³

Affiliation:

1. D. Bieber, S. W. Ramer, C.-Y. Wu, R. Fernandez, G. K. Schoolnik, Department of Medicine, Division of Infectious Diseases and Geographical Medicine, and Department of Microbiology and Immunology, Stanford Program for Vaccine Research, Stanford University Medical Center, Stanford, CA 94305 USA.

2. W. J. Murray, Department of Biological Sciences, San Jose State University, San Jose, CA 95912 USA.

3. T. Tobe, Department of Bacteriology, Institute of Medicine, University of Tokyo, Tokyo, Japan.

Abstract

Type IV bundle-forming pili of enteropathogenic Escherichia coli are required for the localized adherence and autoaggregation phenotypes. Whether these pili are also required for virulence was tested in volunteers by inactivating bfpA or bfpT ( perA ) encoding, respectively, the pilus subunit and the bfp operon transcriptional activator. Both mutants caused significantly less diarrhea. Mutation of the bfpF nucleotide-binding domain caused increased piliation, enhanced localized adherence, and abolished the twitching motility–dispersal phase of the autoaggregation phenotype. The bfpF mutant colonized the human intestine but was about 200-fold less virulent. Thus, BfpF is required for dispersal from the bacterial aggregate and for full virulence.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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