Developmental barcoding of whole mouse via homing CRISPR-Reference-Cited by-同舟云学术

Developmental barcoding of whole mouse via homing CRISPR

Published:2018-08-31 Issue:6405 Volume:361 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Kalhor Reza¹²^ORCID,Kalhor Kian³^ORCID,Mejia Leo¹^ORCID,Leeper Kathleen²^ORCID,Graveline Amanda²^ORCID,Mali Prashant⁴,Church George M.¹²^ORCID

Affiliation:

1. Department of Genetics, Harvard Medical School, Boston, MA, USA.

2. Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.

3. Department of Electrical Engineering, Sharif University of Technology, Tehran, Iran.

4. Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Abstract

Lineage tracing in mouse using CRISPR A homing guide RNA (hgRNA) that directs CRISPR-Cas9 to its own DNA locus can diversify its sequence and act as an expressed genetic barcode. Kalhor et al. engineered a mouse line carrying 60 independent loci of hgRNAs, thus generating a large number of unique barcodes in various embryonic and extraembryonic tissues in fully developed mice. This method demonstrates lineage tracing from the very first branches of the development tree up to organogenesis events and was used to elucidate embryonic brain patterning. Science , this issue p. eaat9804

Funder

National Institutes of Health

Intelligence Advanced Research Projects Activity

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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