Developmental barcoding of whole mouse via homing CRISPR

Author:

Kalhor Reza12ORCID,Kalhor Kian3ORCID,Mejia Leo1ORCID,Leeper Kathleen2ORCID,Graveline Amanda2ORCID,Mali Prashant4,Church George M.12ORCID

Affiliation:

1. Department of Genetics, Harvard Medical School, Boston, MA, USA.

2. Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.

3. Department of Electrical Engineering, Sharif University of Technology, Tehran, Iran.

4. Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Abstract

Lineage tracing in mouse using CRISPR A homing guide RNA (hgRNA) that directs CRISPR-Cas9 to its own DNA locus can diversify its sequence and act as an expressed genetic barcode. Kalhor et al. engineered a mouse line carrying 60 independent loci of hgRNAs, thus generating a large number of unique barcodes in various embryonic and extraembryonic tissues in fully developed mice. This method demonstrates lineage tracing from the very first branches of the development tree up to organogenesis events and was used to elucidate embryonic brain patterning. Science , this issue p. eaat9804

Funder

National Institutes of Health

Intelligence Advanced Research Projects Activity

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference84 articles.

1. M. H. Kaufman The Atlas of Mouse Development (Elsevier 1992).

2. The embryonic cell lineage of the nematode Caenorhabditis elegans

3. D. L. Riddle T. Blumenthal B. J. Meyer J. R. Priess in C. elegans II (Cold Spring Harbor Laboratory Press ed. 2 1997).

4. Cell Lineage Analysis by Intracellular Injection of a Tracer Enzyme

5. Widespread Dispersion of Neuronal Clones Across Functional Regions of the Cerebral Cortex

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