Multiplexed protein-DNA cross-linking: Scrunching in transcription start site selection-Reference-Cited by-同舟云学术

Multiplexed protein-DNA cross-linking: Scrunching in transcription start site selection

Published:2016-03-04 Issue:6277 Volume:351 Page:1090-1093
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Winkelman Jared T.¹²³⁴,Vvedenskaya Irina O.¹³,Zhang Yuanchao¹⁵,Zhang Yu²³,Bird Jeremy G.¹²³,Taylor Deanne M.¹⁵⁶⁷,Gourse Richard L.⁴,Ebright Richard H.²³,Nickels Bryce E.¹³

Affiliation:

1. Department of Genetics, Rutgers University, Piscataway, NJ 08854, USA.

2. Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA.

3. Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA.

4. Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53705, USA.

5. Department of Biomedical and Health Informatics, Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USA.

6. Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

7. Department of Obstetrics, Gynecology and Reproductive Sciences, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA.

Abstract

Choosing where to start transcription The RNA polymerase enzyme complex binds to the promoter of a gene and separates the two DNA strands. The subsequently formed “transcription bubble” is required for RNA synthesis to begin. How RNA polymerase chooses the exact DNA base at which it will start transcription has been unclear. Winkelman et al. show that a control element upstream of the start site is involved in helping RNA polymerase make this choice in bacteria. Start site selection involves promoter scrunching, where a stationary RNA polymerase unwinds and pulls DNA through the active site, scrunching the DNA of the transcription bubble. Science , this issue p. 1090

Funder

NIH

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference23 articles.

1. Initial Events in Bacterial Transcription Initiation

2. Massively Systematic Transcript End Readout, “MASTER”: Transcription Start Site Selection, Transcriptional Slippage, and Transcript Yields

3. Start site selection atlacUV5 promoter affected by the sequence context around the initiation sites

4. Effects of transcriptional start site sequence and position on nucleotide-sensitive selection of alternative start sites at the pyrC promoter in Escherichia coli;Liu J.;J. Bacteriol.,1994

5. Factors Affecting Start Site Selection at the Escherichia coli fis Promoter

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