Programmable molecular transport achieved by engineering protein motors to move on DNA nanotubes

Author:

Ibusuki Ryota1ORCID,Morishita Tatsuya1,Furuta Akane23,Nakayama Shintaro1,Yoshio Maki3,Kojima Hiroaki3,Oiwa Kazuhiro13ORCID,Furuta Ken’ya3ORCID

Affiliation:

1. Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo 678-1297, Japan.

2. Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo 102-0083, Japan.

3. Advanced ICT Research Institute, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.

Abstract

Intracellular transport is the basis of microscale logistics within cells and is powered by biomolecular motors. Mimicking transport for in vitro applications has been widely studied; however, the inflexibility in track design and control has hindered practical applications. Here, we developed protein-based motors that move on DNA nanotubes by combining a biomolecular motor dynein and DNA binding proteins. The new motors and DNA-based nanoarchitectures enabled us to arrange the binding sites on the track, locally control the direction of movement, and achieve multiplexed cargo transport by different motors. The integration of these technologies realized microscale cargo sorters and integrators that automatically transport molecules as programmed in DNA sequences on a branched DNA nanotube. Our system should provide a versatile, controllable platform for future applications.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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