CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells-Reference-Cited by-同舟云学术

CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells

Published:2017-01-06 Issue:6320 Volume:355 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Liu S. John¹²,Horlbeck Max A.³⁴⁵⁶,Cho Seung Woo⁷,Birk Harjus S.¹²,Malatesta Martina¹²,He Daniel¹²,Attenello Frank J.¹²,Villalta Jacqueline E.³⁴⁵⁶,Cho Min Y.³⁴⁵⁶,Chen Yuwen³⁴⁵⁶,Mandegar Mohammad A.³,Olvera Michael P.³,Gilbert Luke A.³⁴⁵⁶,Conklin Bruce R.³⁸⁹,Chang Howard Y.⁷,Weissman Jonathan S.³⁴⁵⁶,Lim Daniel A.¹²¹⁰

Affiliation:

1. Department of Neurological Surgery, University of California, San Francisco, CA 94143, USA.

2. Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA.

3. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA.

4. Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA.

5. California Institute for Quantitative Biomedical Research, University of California, San Francisco, CA 94143, USA.

6. Center for RNA Systems Biology, University of California, San Francisco, CA 94143, USA.

7. Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA, USA.

8. Deparment of Medicine, University of California, San Francisco, CA 94143, USA.

9. Gladstone Institute of Cardiovascular Disease, San Francisco, CA, USA.

10. San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA.

Abstract

A very focused function for lncRNAs The human genome generates many thousands of long noncoding RNAs (lncRNAs). A very small number of lncRNAs have been shown to be functional. Liu et al. carried out a large-scale CRISPR-based screen to assess the function of ∼17,000 lncRNAs in seven different human cell lines. A considerable number (∼500) of the tested lncRNAs influenced cell growth, suggesting biological function. In almost all cases, though, the function was highly cell type—specific, often limited to just one cell type. Science , this issue p. 10.1126/science.aah7111

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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