Empirical Analysis of Transcriptional Activity in the
Arabidopsis
Genome
Author:
Yamada Kayoko12345, Lim Jun12345, Dale Joseph M.12345, Chen Huaming12345, Shinn Paul12345, Palm Curtis J.12345, Southwick Audrey M.12345, Wu Hank C.12345, Kim Christopher12345, Nguyen Michelle12345, Pham Paul12345, Cheuk Rosa12345, Karlin-Newmann George12345, Liu Shirley X.12345, Lam Bao12345, Sakano Hitomi12345, Wu Troy12345, Yu Guixia12345, Miranda Molly12345, Quach Hong L.12345, Tripp Matthew12345, Chang Charlie H.12345, Lee Jeong M.12345, Toriumi Mitsue12345, Chan Marie M. H.12345, Tang Carolyn C.12345, Onodera Courtney S.12345, Deng Justine M.12345, Akiyama Kenji12345, Ansari Yasser12345, Arakawa Takahiro12345, Banh Jenny12345, Banno Fumika12345, Bowser Leah12345, Brooks Shelise12345, Carninci Piero12345, Chao Qimin12345, Choy Nathan12345, Enju Akiko12345, Goldsmith Andrew D.12345, Gurjal Mani12345, Hansen Nancy F.12345, Hayashizaki Yoshihide12345, Johnson-Hopson Chanda12345, Hsuan Vickie W.12345, Iida Kei12345, Karnes Meagan12345, Khan Shehnaz12345, Koesema Eric12345, Ishida Junko12345, Jiang Paul X.12345, Jones Ted12345, Kawai Jun12345, Kamiya Asako12345, Meyers Cristina12345, Nakajima Maiko12345, Narusaka Mari12345, Seki Motoaki12345, Sakurai Tetsuya12345, Satou Masakazu12345, Tamse Racquel12345, Vaysberg Maria12345, Wallender Erika K.12345, Wong Cecilia12345, Yamamura Yuki12345, Yuan Shiaulou12345, Shinozaki Kazuo12345, Davis Ronald W.12345, Theologis Athanasios12345, Ecker Joseph R.12345
Affiliation:
1. Plant Gene Expression Center, Albany, CA 94710, USA. 2. Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA. 3. The Arabidopsis thaliana Genome Center, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. 4. Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA. 5. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, Tsukuba 305-0074, Japan.
Abstract
Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant
Arabidopsis
. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of â¼30% of the
Arabidopsis
ORFeome as a resource for global functional experimentation of the plant proteome.
Publisher
American Association for the Advancement of Science (AAAS)
Subject
Multidisciplinary
Cited by
785 articles.
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