Affiliation:
1. Institute for Genetics, University of Cologne, Weyertal 121, D-50931 Cologne, Germany.
Abstract
In antibody responses, B cells switch from the expression of immunoglobulin (Ig) μ and δ heavy (H) chains to that of other Ig classes (α, γ, or ɛ), each with a distinct effector function. Membrane-bound forms of α, γ, and ɛ, but not μ and δ, have highly conserved cytoplasmic tails. Mutant mice unable to express membrane γ1 H chains or producing tailless γ1 H chains failed to generate efficient IgG1 responses and IgG1 memory. H chain membrane expression after class switching is thus required for these functions, and class switching equips the B cell antigen receptor with a regulatory cytoplasmic tail that naı̈ve B cells lack.
Publisher
American Association for the Advancement of Science (AAAS)
Reference42 articles.
1. Antigen Receptors on B Lymphocytes
2. Esser C., Radbruch A., ibid. 8, 717 (1990).
3. To generate the gene-targeting vector the codon encoding the fourth amino acid (TGG tryptophan) of the IgG1 cytoplasmic tail was converted into a stop codon (TGA) by polymerase chain reaction–mediated mutagenesis. A neo r gene flanked by two loxP sites [
4. Rearrangement-Enhancing Element Upstream of the Mouse Immunoglobulin Kappa Chain J Cluster
5. ] was placed downstream of the stop codon. The third loxP site was inserted 5′ of the two transmembrane exons. A 7.3-kb DNA segment including the exons encoding the extracellular and transmembrane part of the mIgG1 constant region [
Cited by
125 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献