1. D. J. Bewley and M. Black Seeds: Physiology of Development and Germination (Plenum New York ed. 2 1994) chap. 1; A. Vitale and R. Bollini in Seed Development and Germination J. Kigel and G. Galili Eds. (Dekker New York 1995) pp. 73–102; M. Miquel and J. Browse in ibid. pp. 169–193.
2. Mansfield S. G., Briarty L. G., Can. J. Bot. 70, 151 (1992).
3. The cDNA of the Arabidopsis prohibitin gene Atphb1 (L. Sun and H. M. Goodman) in an antisense orientation was inserted into pBI121 (Clontech) between the Sac I and Bam HI sites to replaced the β-glucuronidase coding region. One of 49 C24 transgenic lines showed the shrunken seed phenotype ( sse1 ). Northern (RNA) blot analysis with an Atphb1 cDNA bottom-strand probe showed that the Atphb1 mRNA level in this line is similar to that of the wild type.
4. The transgenic line was propagated by self-pollination for four generations (to T5). Ten round seeds were grown from each generation and each produced 90% shrunken seeds.
5. T2 plants derived from round seeds were crossed reciprocally to wild-type C24 plants. All F 1 seeds were round. The numbers of shrunken/round F 2 seeds in six single siliques were 11/39 15/36 12/39 10/42 11/36 and 10/40; these numbers are consistent with an expected segregation ratio of 1:3 (χ 2 = 0.64 P > 0.1).