Structure of the activated human minor spliceosome-Reference-Cited by-同舟云学术

Structure of the activated human minor spliceosome

Published:2021-03-19 Issue:6535 Volume:371 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Bai Rui¹²³^ORCID,Wan Ruixue¹²³^ORCID,Wang Lin⁴^ORCID,Xu Kui⁴^ORCID,Zhang Qiangfeng⁴^ORCID,Lei Jianlin⁴⁵^ORCID,Shi Yigong¹²³⁴^ORCID

Affiliation:

1. Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Xihu District, Hangzhou 310024, Zhejiang Province, China.

2. Westlake Laboratory of Life Sciences and Biomedicine, Xihu District, Hangzhou 310024, Zhejiang Province, China.

3. Institute of Biology, Westlake Institute for Advanced Study, Xihu District, Hangzhou 310024, Zhejiang Province, China.

4. Beijing Advanced Innovation Center for Structural Biology and Beijing Frontier Research Center for Biological Structure, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.

5. Technology Center for Protein Sciences, Ministry of Education Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.

Abstract

Atomic structure of the minor spliceosome About 1% of the human genome contains the so-called U12-type introns, which are spliced by the minor spliceosome. Compared with the major spliceosome, the composition, assembly, functional states, activation, regulation, and structure of the minor spliceosome have been enigmatic. Bai et al. assembled the activated human minor spliceosome in an in vitro U12-type intron-splicing assay and determined the structure by cryo–electron microscopy at 2.9-angstrom resolution. They found a number of exciting and unanticipated features, including five new proteins that play an important role in the activated minor spliceosome. Science , this issue p. eabg0879

Funder

National Natural Science Foundation of China

China Postdoctoral Science Foundation

National Postdoctoral Program for Innovative Talents of China

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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