Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation-Reference-Cited by-同舟云学术

Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation

Published:2021-11-26 Issue:6571 Volume:374 Page:1152-1157
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Wang Qian¹^ORCID,Xue Yan²^ORCID,Zhang Laixing³^ORCID,Zhong Zhenhui²^ORCID,Feng Suhua²,Wang Changshi¹^ORCID,Xiao Lifan¹^ORCID,Yang Zhenlin¹,Harris C. Jake⁴^ORCID,Wu Zhe¹,Zhai Jixian¹^ORCID,Yang Maojun³^ORCID,Li Sisi⁵^ORCID,Jacobsen Steven E.²⁶^ORCID,Du Jiamu¹^ORCID

Affiliation:

1. Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, School of Life Science, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.

2. Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.

3. Ministry of Education Key Laboratory of Protein Science, Tsinghua-Peking Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

4. Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK.

5. Department of Biochemistry and Molecular Biology, International Cancer Center, Shenzhen University Health Science Center, Shenzhen 518060, China.

6. Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, USA.

Abstract

Ready to dice In the biogenesis of small RNAs, the Dicer family endonucleases act as a molecular ruler to cut the substrate RNA into defined lengths. Wang et al . report a structure of the dicing-competent state of plant DICER LIKE PROTEIN 3 (DCL3) in complex with a pre–small interfering RNA (pre-siRNA). Toward one end of the pre-siRNA, DCL3 uses a positively charged pocket and an aromatic cap to specifically recognize the 5′-phosphorylated adenosine of the guide strand and the 3′ overhang of the complementary strand, respectively. On the other end, the paired ribonuclease III domains of DCL3 cut both strands of the RNA, determining the precise length of the product small RNA. —DJ

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference45 articles.

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