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2. TheSwiss CheeseMutant Causes Glial Hyperwrapping and Brain Degeneration inDrosophila
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4. Drosophila lines carrying P{ lacZ w + } were screened for mutations causing reduced life-span at 29°C. Candidates showing reduced life-span compared with the parent strain were examined after aging (but before death) to identify those with brain degeneration (3). The bubblegum mutant showed such degeneration and was selected for further characterization.
5. Fly heads were prepared by fixation in 1% paraformaldehyde plus 1% glutaraldehyde postfixation in 1% osmium tetroxide dehydration in an ethanol series and embedding in Epon 812. For light microscopy 1-μm sections were stained with 1% toluidine blue plus 1% Borax. For electron microscopy ultrathin sections (80 nm) were examined with a Philips 201 electron microscope at 60 kV.