Affiliation:
1. Department of Molecular and Cell Biology, Division of Genetics, 401 Barker Hall, University of California, Berkeley, CA 94720, USA.
Abstract
Silencing of transcription in
Saccharomyces cerevisiae
has several links to DNA replication, including a role for the origin recognition complex (ORC), the DNA replication initiator, in both processes. In addition, the establishment of silencing at the
HML
and
HMR
loci requires cells to pass through the S phase of the cell cycle. Passage through S phase was required for silencing of
HMR
even under conditions in which ORC itself was no longer required. The requirement for ORC in silencing of
HMR
could be bypassed by tethering the Sir1 protein to the
HMR-E
silencer. However, ORC had a Sir1-independent role in transcriptional silencing at telomeres. Thus, the role of ORC in silencing was separable from its role in initiation, and the role of S phase in silencing was independent of replication initiation at the silencers.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
143 articles.
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