ATP-dependent force generation and membrane scission by ESCRT-III and Vps4-Reference-Cited by-同舟云学术

ATP-dependent force generation and membrane scission by ESCRT-III and Vps4

Published:2018-12-21 Issue:6421 Volume:362 Page:1423-1428
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Schöneberg Johannes¹²³^ORCID,Pavlin Mark Remec²⁴,Yan Shannon¹²^ORCID,Righini Maurizio⁵^ORCID,Lee Il-Hyung¹²^ORCID,Carlson Lars-Anders¹²,Bahrami Amir Houshang³^ORCID,Goldman Daniel H.²⁶⁵,Ren Xuefeng¹²^ORCID,Hummer Gerhard³⁷^ORCID,Bustamante Carlos¹²⁴⁶⁵^ORCID,Hurley James H.¹²⁴⁸^ORCID

Affiliation:

1. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

2. California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA.

3. Department of Theoretical Biophysics, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany.

4. Graduate Group in Biophysics, University of California, Berkeley, Berkeley, CA 94720, USA.

5. Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.

6. Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA.

7. Institute of Biophysics, Goethe University, Frankfurt/M, Germany.

8. Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Abstract

Membrane scission by ESCRTs The ESCRT protein complexes are essential for cell division, the release of HIV from infected cells via budding, and other cell processes involving the scission of narrow membrane necks from their inner surface. The unusual inside-directed membrane cutting has made it hard to recapitulate this reaction and understand its mechanism. Schöneberg et al. encapsulated ESCRTs inside lipid vesicles and used optical tweezers to pull out membrane nanotubes. In the presence of adenosine triphosphate, clusters of ESCRTs generated force and constricted the nanotube, eventually severing it. This approach provides a window into the molecular mechanisms involved in the activities of ESCRTs. Science , this issue p. 1423

Funder

National Science Foundation

Howard Hughes Medical Institute

National Institute of Allergy and Infectious Diseases

Max Planck Society

Marie Skłodowska- Curie Fellowship

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference37 articles.

1. Membrane Fission Reactions of the Mammalian ESCRT Pathway