Systematic humanization of yeast genes reveals conserved functions and genetic modularity

Abstract

Staying the same across a billion years How far across evolution do families of genes retain their function? Yeast and humans are separated by roughly a billion years of evolutionary history, and yet genes from one can substitute for orthologous genes in the other. To study this effect systematically, Kachroo et al. replaced over 400 essential yeast genes with their human orthologs. Roughly half of the human genes could functionally replace their yeast counterparts. Genes being in the same pathway was as important as sequence or expression similarity in determining replaceability. Science , this issue p. 921