A Conformational Switch in Escherichia coli 16 S Ribosomal RNA During Decoding of Messenger RNA-Reference-Cited by-同舟云学术

A Conformational Switch in Escherichia coli 16 S Ribosomal RNA During Decoding of Messenger RNA

Published:1997-08-29 Issue:5330 Volume:277 Page:1262-1267
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Lodmell J. Stephen¹,Dahlberg Albert E.¹

Affiliation:

1. The authors are in the Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA.

Abstract

Direct evidence is presented for a conformational switch in 16 S ribosomal RNA (rRNA) that affects tRNA binding to the ribosome and decoding of messenger RNA (mRNA). These data support the hypothesis that dynamic changes in rRNA structure occur during translation. The switch involves two alternating base-paired arrangements apparently facilitated by ribosomal proteins S5 and S12, and produces significant changes in the rRNA structure. Chemical probing shows reciprocal enhancements or protections at sites in 16 S rRNA that are at or very near sites that were previously crosslinked to mRNA. These data indicate that the switch affects codon-anticodon arrangement and proper selection of tRNA at the ribosomal A site, and that the switch is a fundamental mechanism in all ribosomes.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference79 articles.

1. R. F. Gesteland and J. F. Atkins Eds. The RNA World (Cold Spring Harbor Laboratory Press Plainview NY 1993).

2. Unusual Resistance of Peptidyl Transferase to Protein Extraction Procedures

3. Shine J., Dalgarno L., Proc. Natl. Acad. Sci. U.S.A. 71, 1342 (1974);

4. Hui A., de Boer H. A., ibid. 84, 4762 (1987);

5. ; W. F. Jacob M. Santer A. E. Dahlberg ibid. p. 4757; R. A. Calogero C. L. Pon M. A. Canonaco C. O. Gualerzi ibid. 85 6427 (1988).

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